Comparative study of direct and indirect methods for estimation of serum HDL cholesterol

Dr Ashwini K, Dr Syed AJ

Background: High density lipoprotein cholesterol (HDLc) can be estimated in two ways. The first one is direct, homogenous assay method which is easier but more expensive. The second one is indirect precipitation method is more time consuming but less expensive. Methods: A total of 200 fasting serum samples, both sexes were analyzed by both methods. The mean values along with their standard deviation were tabulated and were compared by students t test. Result: No significant difference in the mean levels of HDLc was observed between both methods irrespective of serum total cholesterol levels. Conclusion: In a developing country like India, where a lot of people belong to below poverty line, estimation of HDLc can be done by indirect method which is cost effective without compromising the quality of the results obtained.

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