Demonstrating glycogen at various stages of developing human liver

DR. HASHMI INTKHAB C.

Abstract: Ninety five percent of all the glycogen stored in the body is found in the liver and muscles. Liver glycogen serves as a major source of blood glucose and is of utmost importance for supply of glucose to other organs, especially the brain. The metabolism of liver glycogen will depend on the nutritional state of the animal/human. Forty human fetuses (19 males and 21 females) of different gestational ages ranging from 12th to 36th gestational weeks were procured for the research work. Slides of liver were stained with periodic acid Schiff (PAS) stain to demonstrate the glycogen. The Diastase PAS stain was used for the demonstration of glycogen in combination with the PAS stain. Glycogen granules appeared first at 15th to 16th weeks of gestation in the form of fine granules; thereafter they increase in size till birth.

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